Table 3.
Protein enrichments by immunoprecipitations
| Protein enrichments (%)
|
||||
|---|---|---|---|---|
| WT
(%)
|
chc1 (%)
|
|||
| αGST | αClc1p | αGST | αClc1p | |
| Clc1p | <0.5 | >60 | ND | ND |
| Chc1p | <0.5 | 60 | ND | ND |
| Apl1p | <0.5 | <0.5 | <0.5 | <0.5 |
| Apl2p | <0.5 | 6 | <0.5 | <0.5 |
| Vps10p-HA | <0.5 | 6 | <0.5 | 1.5 |
Each value represents protein immunoprecipitation with αGST and αClc1p antibodies, respectively, in percentage of the total HSP fraction with a standard error of ∼20%. Quantification was performed from three independent immunoprecipitation experiments and determined by using Phosphorimager (Molecular Dynamics).
ND, not determined.