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An increase in production of GP73 was not observed in Hep3B cells transfected with the GDP-Fuc Tr gene. Immunoblotting of GP73 was performed for Hep3B cells transfected with GDP-Fuc Tr. Lanes 1 and 2 are control and Fut8 transfectant, respectively. Lanes 3 and 4, independent clones of GDP-Fuc Tr transfectants. Lectin blotting with AAL was performed, using the same samples. Coomassie brilliant blue (CBB) staining indicated that equal amounts of protein were loaded in the lanes.
