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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Hum Pathol. 2011 Feb 2;42(6):873–881. doi: 10.1016/j.humpath.2010.10.006

Figure 1.

Figure 1

Detection of epithelial areas using stereological “phantoms”, a dense array of circles randomly placed on the image. Each phantom becomes a distinct object that can be sorted according to its content of cytoplasm (cytokeratin), nucleus (DAPI) and p27. Phantoms containing little or no epithelium are discarded by gating in a scatterplot.