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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Reprod Dev. 2011 Apr 8;78(5):306–317. doi: 10.1002/mrd.21306

Figure 1.

Figure 1

DNA Methyltransferase expression in aging donor cell lines. a) Dnmt expression in aging primary donor fibroblasts. Primary donor fibroblasts used as nuclear donors were cultured to senescence and RNA collected at each passage was analyzed for methyltransferase expression using quantitative RT-PCR. Error bars represent the SEM and * denote statistical significance P<0.01. b) Dnmt expression in aging cumulus cells. Primary cumulus cells were obtained from isolated cumulus-oocyte complexes and cultured to senescence. Measurements of transcripts encoding bovine Dnmts were measured using q-RT-PCR. Samples were normalized to the geometric mean of GAPDH, YWHAZ & SDHA. Error bars represent the SEM and * denote statistical significance P<0.01.