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. Author manuscript; available in PMC: 2012 Jun 3.
Published in final edited form as: J Mol Biol. 2011 Apr 9;409(2):225–237. doi: 10.1016/j.jmb.2011.03.072

Figure 1.

Figure 1

The MS2 single chain coat protein is broadly tolerant of random sequence peptide insertions in the 13/14, 13/16 and 12/17 as indicated by the presence of VLPs in crude cell lysates of a number of individual clones picked randomly from the three different peptide 8-mer libraries. The top panel shows gels stained with ethidium bromide. In the bottom panel, the gels were blotted to nitrocellulose and probed with anti-MS2 serum. Lanes marked pUCter3 and p2MCTK3 denote control lysates that respectively lack and contain MS2 VLPs lacking an inserted peptide.