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. Author manuscript; available in PMC: 2012 Jun 3.
Published in final edited form as: J Mol Biol. 2011 Apr 9;409(2):225–237. doi: 10.1016/j.jmb.2011.03.072

Figure 2.

Figure 2

The basic features of the plasmids, pDSP1 and pDSP62, used in this study for library construction and synthesis of VLPs for affinity selection.The plasmid pDSP1 is suitable for library construction by simple insertion of a Sal I – Bam HI fragment to which random sequences have been attached by PCR. On the other hand, pDSP62 allows extension of a mutagenic primer on a single-stranded circular template to introduce insertions by the site-directed mutagenesis method of Kunkel et al. 15 as implemented for library construction in filamentous phage by Sidhu et al. 14. The upstream half of the single-chain dimer contains a sufficient number of silent mutations (codon juggled) that a mutagenic primer can be directed to anneal specifically to the downstream half.