Effect of SHU9119 on food intake (mean ± S.E.M.) in satiated wild type, MC4KO, and MC3KO littermate mice (n=10–12 per group). A) In the wild type mice the 1.0 and 3.0 nmol dose statistically increased food intake relative to saline starting at t=7h and t= 5h, respectively, and continued to remain statistically significant up to the 24h post injection time point (*p<0.05). B) In the MC4KO and MC3KO mice, no statistically significant changes in food intake relative to saline was observed.