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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Virol Methods. 2011 Mar 17;174(1-2):12–21. doi: 10.1016/j.jviromet.2011.03.012

Table 2. Comparison of rKSHV.219 stocks prepared from inducible Vero cells (iVero.219), harboring rKSHV.219, and iSLK.219.

4 independent stocks were compared for their infectious units and particle numbers, determined by real-time QPCR. Each stock was prepared by concentrating virus-containing culture supernatants by resuspending virus pellets in fresh medium of the 1/100 original volume. The number of WT KSHV particles, prepared from BCBL-1 cells, is shown for comparison.

Infectious
Units/ml		 Particles/ml Particle/IU
ratio
rKSHV stock from Vero. 219 Exp #1 1.2*106/ml 4.99*108/ml 415.8
Exp #2 34.5*106/ml 60.3*108/ml 174.7
Exp #3 50.1*106/ml 450.2*108/ml 898.6
Exp #4 146.1*106/ml 560.1*108/ml 383.3
rKSHV stock from SLK. 219 Exp #1 1.1*106/ml 0.88*108/ml 80.3
Exp #2 19.7*106/ml 86.0*108/ml 436.5
Exp #3 30.1*106/ml 121.1*108/ml 402.3
Exp #4 111.3*106/ml 352.1*108/ml 316.3

cf) BCBL-1 virus : 873.5*108/ml