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C-mannosylation of RNase 2.4. RNase 2.4 was expressed in either CHO-K1 (A) or Lec35.1 (B) cells, purified from the conditioned medium, digested with thermolysin at 75°C, and the peptides were fractionated by reversed phase C18 HPLC. Only the portion of the chromatogram where peptides from the N terminus of the protein eluted has been shown. Peak b contains the C-mannosylated peptide [FT(C2-Man-)WAQW], whereas peaks a and c contain two forms of the unmodified peptide, TWAQW and FTWAQW.
