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Glucosylation of Man9GlcNAc2-P-P-dolichol in vivo depends upon the level of expression of Lec35 mRNA. CHO-K1 (A and B), Lec 35.1 (C and D), 6C (E and F), or 10A cells (G and H) were grown in the absence (A, C, E, and G) or presence (B, D, F, and H) of 10 ng/ml doxycyclin for 4 d, and labeled with 1 mCi/ml [2-3H]mannose for 15 min. Oligosaccharides were obtained from the LLO fraction by mild acid hydrolysis and analyzed by HPLC.
