Figure 6.

Mild perturbation efficiently corrects defective glycosylation in Lec35 cells. Lec35.1 (A and C) and Lec15.2 (B and D) cells were incubated with [³H]mannose to label endogenous Man₅GlcNAc₂-P-P-dolichol. Cells were treated in the absence (A and B; control) or presence (C and D; TX-100) of 0.05% (wt/vol) Triton X-100, and then incubated with 0.1 mM unlabeled GDP-mannose. The oligosaccharides were recovered and compared with known standards, as indicated, by HPLC. Note that Man₅GlcNAc₂-P-P-dolichol in treated Lec15 cells (D) is converted to Glc₃Man₅GlcNAc₂-P-P-dolichol, but additional mannose residues are not added.