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Mannose-P-nerol is not a mannose donor with SLO-treated Lec35 cells. Lec35.1 cells were incubated with [3H]mannose and treated with SLO as in Figure 8. The cells were then incubated in buffer containing 100 μM unlabeled GDP-mannose alone (A), with MPC (B), with MPN (C), or with MPC plus MPN (D), each at a concentration of 200 μM. LLO were then analyzed by HPLC.
