Figure 6.
POMC-DsRed+ neurons in the arcuate nucleus and the pituitary show β-endorphin immunoreactivity. A: β-endorphin immunoreactivity (green) was limited to neuronal fibers and showed only weak immunoreactivity in cell bodies of DsRed+ hypothalamic neurons (magenta). B: Inhibiting axonal transport by colchicine increased β-endorphin immunoreactivity in the soma of DsRed+ POMC neurons. In the pituitary, DsRed+ cell (magenta) are immunolabeled for β-endorphin (C) and ACTH (D) (both green) without colchicine treatment. Scale bars: 20µm.