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. Author manuscript; available in PMC: 2011 May 17.
Published in final edited form as: Dev Cell. 2006 Oct;11(4):575–582. doi: 10.1016/j.devcel.2006.08.010

Figure 3. MEK Is Essential for DNA Damage-Induced Checkpoint Response.

Figure 3

(A) Embryos (0–1 hr AED) were treated with low-dose X-ray radiation (150 rad), allowed to develop for 35 min at 25°C, and then fixed and stained with propidium iodide (red) and antibodies for α-Tubulin (cyan) and phospho-Histone H3 (pH3; green).

(B) Interphase index of embryos 35 min following the treatment in (A). The interphase index is defined as the percentage of pH3-negative embryos of the total. “n” denotes the number of embryos counted. Error bars represent standard deviation.

(C) Immediately following X-ray irradiation (within 10 min) as in (A), embryo lysates were subjected to SDS-PAGE and blotted sequentially with indicated antibodies.

(D) 60 min following irradiation as in (A), embryos were fixed and stained with propidium iodide (red) and anti-pH3 (green). The arrow points to a second nuclear layer formed by falling nuclei (middle). Note the unusually large and fragmented DNA (pH3-positive) in the MEK embryo.

(E) Freshly laid eggs (0–1 hr AED) of indicated genotypes were treated with 150 rad X-ray, and then ten embryos were homogenized at each indicated time point. Embryo lysates were subjected to SDS-PAGE and blotted sequentially with indicated antibodies. The membrane was stripped of antibodies between blotting.