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. 2001 Feb;12(2):503–510. doi: 10.1091/mbc.12.2.503

Figure 2.

Figure 2

Figure 2

Dextran (70 kDa) entry during GVBD. Rhodamine dextran (70 kDa) was injected into the cytosol and imaged by a two-photon microscope at 2.5-s intervals. This image sequence was begun 10–15 min after addition of the maturation hormone 1-MA. Timing relative to the first image is shown on each image (minutes:seconds). There is an initial phase of slow entry followed by a massive entry from one region of the nuclear envelope at the time of GVBD (between g and h). The wave front develops a concave shape. See movie “2pgvbd.mov” at the Molecular Biology of the Cell web site. The small dark circle to the right of the GV is an oil drop introduced by microinjection; another oil drop out of the plane of focus accounts for the dark region beneath the GV. Bar, 50 μm. Graph, from this image sequence, the average fluorescence intensity of a 30- × 30-μm region at the center of the GV was determined. The time points corresponding to those shown in the image sequence are indicated by the lower case letters.