Figure 2.

Comparative assessment of pulmonary inflammation in C57B/6 WT and Glrx1^−/− mice after oropharyngeal aspiration of LPS. (A) BALF was collected from WT C57B/6 or Glrx1^−/− mice at time points indicated. Cells were enumerated using an automated cytometer. Data are expressed as mean (± SEM) total cell counts (n = 4–8 mice/group/time point). Actual P values for differences between Glrx1^−/− and WT mice at 16-hour and 24-hour time points are indicated. (B) Cellular differentials in BAL reflect percentages of macrophages (MAC), lymphocytes (LYMPH), and neutrophils (PMN). No statistically significant differences were apparent between WT and Glrx1^−/− groups. (C) Evaluation of cytokines in cell-free BALF via ELISA. Data are expressed as mean pg/ml (± SEM) (n = 4–8 mice/group/time point). *P < 0.05, compared with WT mice (ANOVA).