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. 2010 Jun 17;44(4):548–555. doi: 10.1165/rcmb.2010-0078OC

Figure 4.

Figure 4.

Modulation of protein serotonylation in SMCs in culture by 5-HT transporter (5-HTT), monoamine oxidase (MAO), and TGase. Bovine SMCs were washed with serum-free DMEM and then pretreated with (A) 5-HTT inhibitor, imipramine or fluoxetine (1,10 μmol/L) (B) MAO inhibitor, phenelzine (50 μmol/L), or (C) TGase inhibitor, DSC (200 μmol/L) for 30 minutes. Protein serotonylation was then induced by 5-HT (1 μmol/L) for 30 minutes. Cells lysates underwent electrophoresis, and serotonylation was analyzed by Western blotting with anti–5-HT–BSA conjugate antibody. Bar graphs represent means (±SD) for n = 3. *Significant difference from the untreated controls (P < 0.05); #significant difference from 5-HT–treated cells (P < 0.05).