TABLE 1.
PRIMER SEQUENCES
| Gene | Accession No. | Sequences (5′ → 3′) | Amplicon (bp) | |
|---|---|---|---|---|
| Hmga2 | NM_010441 | Forward | AAGGCAGCAAAAACAAGAGC | 121 |
| Reverse | GCAGGCTTCTTCTGAACGAC | |||
| CBF-A | L36663 | Forward | GGGAAAAATGTTCGTTGGTG | 130 |
| Reverse | CCCTCTTGATCGTCCAGTGT | |||
| Fsp-1 | NM_0113111 | Forward | CTGGGGAAAAGGACAGATGA | 109 |
| Reverse | TGCAGGACAGGAAGACACAG | |||
| E-cadherin | NM_009864.2 | Forward | AGCCATTGCCAAGTACATCC | 133 |
| Reverse | AAAGACCGGCTGGGTAAACT | |||
| PAI-1 | NM_008871 | Forward | AGTCTTTCCGACCAAGAGCA | 105 |
| Reverse | GACAAAGGCTGTGTGGAGGAAG | |||
| Fn1 | NM_010233 | Forward | GTGTAGCACAACTTCCAATTACGAA | 90 |
| Reverse | GGAATTTCCGCCTCGAGTCT |
Primer sequences for quantitative PCR cycling conditions with similar efficiencies to obtain simultaneous amplification in the same run. Sequences were taken from GeneBank; all accession numbers are denoted.