Figure 7.

Adoptive transfer of DC and non-DC splenic cells from Day 14 allergen-naive neonates of OVA-sensitized mothers with CAS challenge of recipients. CD11c⁺ DCs, CD11b⁺ macrophages, and CD4⁺ T cells, as well as DC-depleted splenocytes from offspring of asthmatic (DC, Mac, CD4, and FlowThru, respectively) and normal mothers (not shown except for normal DC control), were transferred to recipients later sensitized to and challenged with CAS. Both airway responsiveness (a) and inflammation (b) remained minimal in recipients of CD11b⁺ macrophages, CD4⁺ T cells, and CD11c-depleted cells from neonates of asthmatic mothers. Values were similar to those obtained from respective control recipients of the same cells from offspring of normal mothers (not shown). At the same time, the effect of DCs remained reproducible, as indicated by increased airway responsiveness and inflammation in the As-DC group (n = 6 per group, *P < 0.05).