Figure 6.

Mesoderm compaction and cohesivity is inhibited by EMAPII, whereas endoderm compaction is not. Aggregate formation was examined in isolated epithelial and mesenchymal cells. Mesenchymal sheet compaction was significantly inhibited by EMAPII (A) (P = 0.014, n = 10, representative experiment, performed 10 times) as compared with controls. Mesenchymal bodies (MBs) subjected to TST were noted to have liquid properties and surface tension that was independent of aggregate size (B) (r² = 0.046). MB cohesivity was markedly decreased in aggregates treated with EMAPII as compared with controls (C) (P = 0.001, n = 10).