Figure 5.

Effect of exogenous di8:0 diacylglycerol administration on diacylglycerol pool sizes. CTY434 cells ± plasmids were grown overnight at 25°C in synthetic dextrose media containing the appropriate nutrients. Optical densities were measured at 600 nm and each strain was diluted to A₆₀₀ of 0.150. Cells were grown for 1 h at 25°C and the medium was subsequently supplemented with di8:0 DAG to a final concentration of 200 μM. Five-milliliter aliquots were removed at the indicated time points and diacylglycerols were extracted and quantified as described in “Experimental Procedures.” (A) CTY434 cells grown without di8:0 DAG supplementation. (B) CTY434 cells grown with di8:0 DAG supplemented to 200 μM di8:0 DAG. (C) CTY434 cells containing high copy plasmids carrying the CPT1 or EPT1 genes supplemented to 200 μM di8:0 DAG for 4 h. Results are the mean of n = 4 experiments. SEs were <15% for each mean.