FIGURE 1. Global gene expression analysis and multidimensional scaling of EBV-transformed lymphoblastoid cell lines (EBV-TM-LCL) used as feeder cells for NK-cell expansion.
(A) Global gene expression analysis: 3 EBV-TM-LCL lots cultured for up to 90 days were analyzed (n = 17). The 12,283 genes remaining after filtering were analyzed by unsupervised hierarchical clustering analysis. No differences were noted between the cultured cell lines. The sample label LCL-3-6-D79 indicates the sixth cell line from EBV-TM-LCL lot 3 with culture duration of 79 days. (B) Unsupervised multidimensional scaling analysis of gene expression data using centered correlation: each spot represents one EBV-TM-LCL sample. No effects of culture duration on EBV-TM-LCL were noted.