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. Author manuscript; available in PMC: 2011 May 17.
Published in final edited form as: Eur J Immunol. 2010 Apr;40(4):1099–1106. doi: 10.1002/eji.200940022

Figure 3. Phenotype of human PB CD4 subsets.

Figure 3

Freshly isolated human PBMCs were stained for CD4, CD25, TNFR2 and additional phenotypic markers. Intracellular expression of CTLA-4 and surface expression of CD45RO, CD45RA, CCR4, CCR7, CD127 and HLA-DR by different subsets of PB CD4 cells, gating on indicated populations were analyzed by FACS. Black filled histogram: antibody staining; grey histogram: isotype control. Numbers in the figures indicate the percentage of gated cells expressing relevant marker. Data shown are representative of at least three separate experiments on different donors with similar results.