ECD spectra of CLN025 at 293 K in A, 0 (black), 1 (violet), 2 (blue), 3 (green), 4 (cyan), 5 (orange) and 6 M GdmCl (red); B, 0 (black), 1 (violet), 2 (maroon), 3 (blue), 4 (green), 5 (cyan), 6 (orange), 7 (magenta) and 8 M urea (red); and C, the mean residue ellipticity of CLN025 at 230 nm in various concentrations of GdmCl (black) and urea (red).