ECD spectra of CLN025 in A, buffer; B, 3 M GdmCl; C, 6 M GdmCl; D, 4 M urea and E, 8 M urea at 293 (black), 303 (violet), 313 (blue), 323 (green), 333 (light green), 343 (orange) and 353 K (red) and F, mean residue ellipticity of CLN025 at 230 nm at various temperatures in buffer (black), 3 M GdmCl (blue), 6 M GdmCl (orange), 4 M urea (green) and 8 M urea (red).