The backbone and side-chains of A, the crystal structure; and the central structures of the most populated cluster of trajectories of MD simulations of CLN025 in B, water at 300 K; C, water at 333 K; D, 3 M GdmCl at 300 K; E, 6 M GdmCl at 300 K; F, 6 M GdmCl at 333 K; G, 4 M urea at 300 K; H, 8 M urea at 300 K; and I, 8 M urea at 333 K. The backbone structure represented by a tube. Turn is green, β-sheet is red and random meander is cyan.