Table 5.
H-bonding residues | Water | 3 M GdmCl | 6 M GdmCl | 4 M Urea | 8 M Urea | |||
---|---|---|---|---|---|---|---|---|
300 K | 333 K | 300 K | 300 K | 333 K | 300 K | 300 K | 333 K | |
Tyr1-Trp9 | 0.45 | 0.64 | 0.04 | 0.00 | 0.00 | 0.780 | 0.10 | 0.24 |
Tyr1-Tyr10 | 0.87 | 0.34 | 0.44 | 1.42 | 1.30 | 0.30 | 0.60 | 0.02 |
Asp3-Glu5 | 0.30 | 0.39 | 0.32 | 0.13 | 0.22 | 0.44 | 0.64 | 0.36 |
Asp3-Thr6 | 1.83 | 1.44 | 1.73 | 1.90 | 1.73 | 1.53 | 1.39 | 0.56 |
Asp3-Gly7 | 0.91 | 0.93 | 0.58 | 0.44 | 0.45 | 1.04 | 0.67 | 0.44 |
Asp3-Thr8 | 0.78 | 0.34 | 0.55 | 1.54 | 1.38 | 0.30 | 0.53 | 0.02 |
Pro4-Gly7 | 0.10 | 0.04 | 0.12 | 0.29 | 0.22 | 0.04 | 0.05 | 0.01 |
Thr6-Thr8 | 0.50 | 0.20 | 0.32 | 0.88 | 0.81 | 0.23 | 0.35 | 0.05 |
Thr6-Trp9 | 0.24 | 0.34 | 0.01 | 0.00 | 0.00 | 0.38 | 0.07 | 0.14 |
Thr8-Tyr10 | 0.10 | 0.05 | 0.10 | 0.17 | 0.17 | 0.05 | 0.05 | 0.04 |
Total | 6.4 | 4.7 | 5.0 | 7.5 | 6.3 | 5.4 | 5.2 | 1.9 |