FIGURE 8.

Analysis of the effect of a pnp deletion and the rneΔ14 and rne-131 alleles on sRNA regulation. (A) Overnight cultures of the cyaR deletion strain (NRD377; wild type), an rneΔ14 mutant (NRD673; rneΔ14), an rne-131 mutant (NRD674; rne-131), a pnp deletion mutant (NRD677; Δpnp), an rneΔ14 Δpnp double mutant (NRD679; Δpnp rneΔ14), and an rne-131 Δpnp double mutant (NRD680; Δpnp rne-131) harboring either a vector or a plasmid expressing CyaR from a lac promoter (pNRD405) were diluted 200-fold in fresh LB containing ampicillin and IPTG. (B) Overnight cultures of the cyaR deletion strain (NRD377; wild type), an rneΔ10 mutant (NRD672; rneΔ10), a pnp deletion mutant (NRD677; Δpnp), and an rneΔ10 Δpnp double mutant (NRD678; Δpnp rneΔ10) harboring either a vector or a plasmid expressing CyaR from a lac promoter (pNRD405) were diluted 200-fold in fresh LB containing ampicillin and IPTG. Strains were grown to an OD₆₀₀ between 0.3 and 0.4. Samples were taken from all cultures, and a β-galactosidase assay was performed on those samples as described by Miller (1992).