FIG. 1.

786-O cells express HIF-2α but not HIF-1α. 786-O (PRC-3) is a VHL-defective renal carcinoma cell line, whereas 786-O (WT-8) cells have been stably transfected with functional VHL (38). RCC-4 is another VHL-mutant renal carcinoma cell line, expressing both HIF-1α and HIF-2α. The RCC-4 (T3-14) cells have been stably transfected with functional VHL. Enhanced expression of HIF-1α mRNA in VHL-transfected RCC-4 cells has previously been observed (55). Hypoxia (H) treatment (1.5% O₂ for 16 h) stabilized HIF-2α protein in 786-O (WT-8) cells and stabilized both HIF-1α and HIF-2α in RCC-4 (T3-14) cells as shown by HIF-1α and HIF-2α Western blots. The ARNT Western blot served as a protein loading control.