. Author manuscript; available in PMC: 2012 May 20.

Published in final edited form as: J Mol Biol. 2011 Apr 1;408(5):987–1000. doi: 10.1016/j.jmb.2011.03.043

Table 2.

Thermodynamic Parameters for Binding Sin3 PAH2 or MRG15 MRG by Wild-type or Mutant Pf1^200–241

Reactants	K_D (μM)	N	ΔH (kcal mol⁻¹)	ΔS (cal mol⁻¹ K⁻¹)
wild-type Pf1 + mSin3A PAH2	2.15 ± 0.45	0.99 ± 0.05	−6.58 ± 0.48	3.9 ± 2.0
Pf1 F210A + mSin3A PAH2	-^a	-^a	-^a	-^a
Pf1 L212A + mSin3A PAH2	2.66 ± 0.46	1.00 ± 0.11	−4.31 ± 0.49	11.0 ± 2.0
Pf1 L213A + mSin3A PAH2	-^a	-^a	-^a	-^a
Pf1 A216V + mSin3A PAH2	-^a	-^a	-^a	-^a
Pf1 N221E + mSin3A PAH2	0.63 ± 0.05	1.16 ± 0.08	−7.67 ± 0.43	2.6 ± 1.6
Pf1 F225A + mSin3A PAH2	1.65 ± 0.43^b	1.12 ± 0.05^b	−4.97 ± 0.34^b	9.8 ± 1.7^b
wild-type Pf1 + mSin3B PAH2	2.93 ± 0.41^b	1.02 ± 0.01^b	−9.64 ± 0.50^b	−6.9 ± 1.8^b
wild-type Pf1 + MRG15 MRG	0.015 ± 0.003	0.82 ± 0.08	−13.10 ± 0.40	−8.0 ± 1.5

no detectable binding, hence could not be quantified

average values from two independent measurements