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. 2011 Apr 21;44(2):103–111. doi: 10.1267/ahc.10038

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© 2011 The Japan Society of Histochemistry and Cytochemistry

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Akt inhibitor treatment suppressed retinal neovascularization but not vessel loss in the murine oxygen-induced retinopathy model. (A) Representative retinal whole-mounts showing area of capillary-free and neovascularization after 5 days of relative oxygen deficiency exposure and intravitreal injections (P12) of Akt inhibitor and PBS. Areas of capillary (gray) and neovascularization (pink) were quantified. Original magnification ×5. (B) The areas of neovascularization and the total areas were quantified. Data are shown as mean±SEM. (PBS, n=10; recombinant Akt, n=15; ***P≤0.001). (C) The capillary-free areas and the total areas were quantified. (D) Histologic sections of P17 retina from PBS-treated (left) and Akt inhibitor-treated (right) eyes of mouse exposed to hyperoxia. Extensive preretinal neovascular tufts were apparent (arrow). (E) The number of neovascular nuclei anterior to the ILM in 6-µm retinal sections was quantified. (PBS, n=10; recombinant Akt, n=15; ***P≤0.001).

Fig. 3 — Akt inhibitor treatment suppressed retinal neovascularization but not vessel loss in the murine oxygen-induced retinopathy model. (A) Representative retinal whole-mounts showing area of capillary-free and neovascularization after 5 days of relative oxygen deficiency exposure and intravitreal injections (P12) of Akt inhibitor and PBS. Areas of capillary (gray) and neovascularization (pink) were quantified. Original magnification ×5. (B) The areas of neovascularization and the total areas were quantified. Data are shown as mean±SEM. (PBS, n=10; recombinant Akt, n=15; ***P≤0.001). (C) The capillary-free areas and the total areas were quantified. (D) Histologic sections of P17 retina from PBS-treated (left) and Akt inhibitor-treated (right) eyes of mouse exposed to hyperoxia. Extensive preretinal neovascular tufts were apparent (arrow). (E) The number of neovascular nuclei anterior to the ILM in 6-µm retinal sections was quantified. (PBS, n=10; recombinant Akt, n=15; ***P≤0.001).