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A schematic drawing shows how to perform “in vivo cryotechnique” (IVCT) for living mouse heart with monitoring electrocardiogram (ECG) (a). Beating heart of an anesthetized mouse on a small animal respirator is exposed after opening the thoracic cavity, which is cryofixed by pouring isopentane-propane cryogen (–193°C) precooled in liquid nitrogen. (b) An example of ECG shows typical P, Q, R, S, and T points, and exact time of stopping with IVCT is indicated by an arrow.
