Fig. 8.

Confocal laser scanning micrographs of double-immunofluorescence staining for IgG1 (a, c; green color) and connexin 43 (b, c; red color) on sections of mouse heart tissues prepared by IVCT at 4 hr after BSA injection. The IgG1 (green color) is immunolocalized in blood vessels (black arrows), interstitium (white arrows), slightly in intercalated discs (large black arrowheads), and t-tubules (small black arrowheads) at 4 hr after BSA injection. At the higher magnification (c; inset), IgG1 and connexin 43 (white arrowhead) are closely immunolocalized in the intercalated discs (large black arrowhead). Bars=10 µm.