Figure 7.
Vam3p and Pep12p do not form quaternary complexes with [Ykt6p + Gos1p + Sft1p], [Ykt6p + Tlg1p + Vti1p] or [Snc2p + Tlg1p + Vti1p]. Mixing assays and immunostaining were preformed as described in MATERIALS AND METHODS. (A) GST-Sft1p was mixed with either the syntaxin (His)6-Sed5p, (His)6-Vam3p, (His)6-Pep12p or (His)6-Tlg1p together with the (His)6-SNAREs Ykt6p and Gos1p. (B) GST-Vti1p was mixed with either (His)6-Sed5p, (His)6-Vam3p or (His)6-Pep12p along with the (His)6-SNAREs Tlg1p and Ykt6p. (C) GST-Snc2p was mixed with (His)6-Sed5p, (His)6-Vam3p, or (His)6-Pep12p together with (His)6-Vti1p and (His)6-Tlg1p. (D) GST-Vam7p was mixed with either (His)6-Vam3p or (His)6-Pep12p together with (His)6-Vti1p and (His)6-Tlg1p. (E) Immunostaining of aliquots of soluble lysates from bacteria expressing the (His)6-SNAREs used in this experiment (∼5% of input).