Figure 9.

gos1Δ and sec22-3 do not display synthetic lethal interactions. Following sporulation of the heterozygous double mutant diploid (SARY327, Table 1), tetrads were dissected on YEPD plates containing 1 M sorbitol and incubated at 25°C for 5 d. (A) Plates shown in B–D were incubated at the indicated temperatures after replica plating (A) for 2 d. Tetrads 2, 4, and 6 are the parental ditype and tetrads 1, 3, 5, and 7 are tetratype. Spores 1a, 3b, 5c, and 7d correspond to the gos1Δ sec22-3 double mutant progeny. The same result was also obtained for the gos1Δ and sec22Δ mutations (data not shown).