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. 2001 Mar;12(3):521–538. doi: 10.1091/mbc.12.3.521

Table 2.

Plasmids used for the expression of recombinant SNARE proteins

Plasmid Description Source
pGST-Sft1 GST-Sft1  (aa 17–78) in pGEX2T Tsui and Banfield, 2000
pGST-Bet1 GST-Bet1  (aa 1–123) in pGEX2T Tsui and Banfield, 2000
pGST-Sed5 GST-Sed5  (aa 1–319) in pGEX2T Banfield lab
pGST-Vti1 GST-Vti1  (aa 1–194) in pGEX2T Tsui and Banfield, 2000
pGST-Snc2 GST-Snc2  (aa 1–93) in pGEX2T This study
pGST-Vam7 GST-Snc2  (aa 1–316) in pGEX2T This study
pSed5-HIS24b Sed5-(His)6  (aa 1–319) in pET24b(+) Tsui and Banfield, 2000
pYkt6-HIS24b Ykt6-(His)6  (aa 2–190) in pET24b(+) Tsui and Banfield, 2000
pGos1-HIS24b Gos1-(His)6  (aa 2–201) in pET24b(+) Tsui and Banfield, 2000
pVti1-HIS24b Vti1-(His)6  (aa 1–194) in pET24b(+) Tsui and Banfield, 2000
pSec22-HIS24b Sec22-(His)6  (aa 1–180) in pET24b(+) Tsui and Banfield, 2000
pBos1-HIS24b Bos1-(His)6  (aa 1–216) in pET24b(+) Tsui and Banfield, 2000
pTIg1-HIS24b Tlg1-(His)6  (aa 1–204) in pET24b(+) This study
pPep12-HIS24b Pep12-(His)6  (aa 1–266) in pET24b(+) This study
pVam3-HIS24b Vam3-(His)6  (aa 1–263) in pET24b(+) This study
pNyv1-HIS24b Nyv1-(His)6  (aa 1–231) in pET24b(+) This study