Table 3.
Multicopy suppression profiles of SNARE deletion strains
| Multicopy plasmidb | Yeast deletion
strainsa
|
||||||
|---|---|---|---|---|---|---|---|
| sft1Δ | bet1Δ | bos1Δ | ykt6Δ | vti1Δ | sft1Δ sec22Δ | sft1Δ snc2Δ | |
| pSFT1 | ++++ | − | − | −c | − | ++++ | ++++ |
| pBET1 | +++c | ++++ | − | − | − | +++ | ++ |
| pBOS1 | − | − | ++++ | − | − | n.d. | n.d. |
| pYKT6 | − | − | − | ++++ | − | n.d. | n.d. |
| pVTI1 | − | − | − | − | ++++ | n.d. | n.d. |
| pSEC22 | − | − | − | − | − | n.d. | n.d. |
| pSED5 | +c | − | − | − | − | n.d. | n.d. |
| pGOS1 | − | − | − | − | − | n.d. | n.d. |
| pSNC2d | ++c | − | − | −c | − | ++ | ++ |
| pTLG1 | − | − | − | − | − | n.d. | n.d. |
n.d. not determined; +/− denotes relative growth rates at 25°C on plates containing 5-FOA where ++++ is wild-type and − is no growth detected.
a
sft1Δ (SARY160), bet1Δ (SARY270), bos1Δ (SARY260), ykt6Δ (SARY158), vti1Δ (SARY296), sft1Δ (SARY160), sec22Δ (SARY323), sft1Δ, snc2Δ (SARY324); see Table 1.
b
Containing genomic DNA fragments encoding the various SNARE genes.
c
Described previously (Tsui and Banfield, 2000).
d
2μ SNC1 does not support the growth of sft1Δ cells.