FIG. 1.

[³H]methyl ester spectra of yeast extracts separated by SDS gel electrophoresis. Yeast cells were labeled in vivo with [³H]AdoMet as explained in Materials and Methods. Subsequently, the cellular extract was fractionated by SDS-10% polyacrylamide gel electrophoresis and the [³H]methanol resulting from hydrolysis of methyl esters was measured as described previously (26). Polypeptide size standards are as follows: phosphorylase b, 97 kDa; bovine serum albumin, 66 kDa; ovalbumin, 45 kDa; carbonic anhydrase, 31 kDa; and soybean trypsin inhibitor, 21 kDa. For RNase treatment, extracts containing approximately 100 μg of protein were incubated with 40 μg of bovine pancreatic RNase A (preincubated by heating at 100°C for 15 min). Symbols: □, wild-type strain BY4742; ▵, F6 (YML014w) deletion mutant HKY101; ○, RNase-treated wild-type strain BY4742; ▴, RNase-treated F6 (YML014w) deletion mutant HKY101.