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. 2003 Dec;23(24):9283–9292. doi: 10.1128/MCB.23.24.9283-9292.2003

FIG. 5.

FIG. 5.

Isolation and characterization of tRNAArg3 and tRNAGlu from in vivo-radiolabeled yeast cells. Total tRNA was isolated from 15 OD600 units of radiolabeled cells as described in Materials and Methods. Biotinylated primers specific for either tRNAArg3 or tRNAGlu were used to purify the tRNAs from either the wild-type parent BY4742 or the HKY101 trm9 deletion strain. Isolated tRNA was digested with P1 nuclease and alkaline phosphatase to generate free nucleosides that were fractionated by HPLC as described in the legend to Fig. 2. One hundred microliters of each fraction was used for measuring the methyl ester radioactivity as described in the legend to Fig. 2. (A) Methyl ester radioactivity from digested tRNAArg3 from the parent (□) and the trm9 deletion (○) strains. (B) Methyl ester radioactivity from digested tRNAGlu from the parent (□) and the trm9 deletion (○) strains.