FIG. 9.

Sensitivity of trm9 deletion strains to paromomycin. (A) Yeast strains (wild-type parent CEN.PK2-1CHKY102 and the trm9Δ HKY102 strain) were grown to log phase at 30°C in the presence of 300 μg of paromomycin/ml. Cells (0.5 OD₆₀₀ unit) were collected and resuspended in 200 μl of sterilized water. Ten microliters of the suspension was diluted in fivefold serial dilutions and plated onto YPD plates, and colonies were allowed to grow for 3 days. (B) Experimental conditions were the same as those described for panel A except that strains were grown at 37°C. (C) Yeast strains containing either the vector pRS316 or the TRM9 expression vector pRS316-TRM9 were grown at 37°C and plated as described above but in a synthetic dextrose medium lacking uracil.