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FIG. 6.

FIG. 6.

MS defines C terminus of ZP3. (A) Microscale electrospray QTOF-MS analysis of an AspN digest of deglycosylated zona pellucida proteins detected quadruply (upper) and triply (lower) charged ions of 330DSSSSQFQIHGPRQWSKLVSRN351 at 40.3 min by reversed-phase liquid chromatography. (B) The triply and quadruply charged ions of the carboxyl terminus peptide at m/z 848.71 and 636.78. (C) The identity of the quadruply charged ion of 330DSSSSQFQIHGPRQWSKLVSRN351 was confirmed by MS/MS.