FIG. 1.

The TGIF HD +20 region. The homeodomain and 20 amino acids carboxyl terminal to it are shown from TGIF (upper group: human, mouse, chicken, Xenopus and zebra fish, shown as h, m, c, x, and z, respectively), TGIF2 (second group: human and mouse, shown as h2 and m2, respectively), Drosophila TGIFa and -b (third group), and a selection of less closely related proteins (lower group). The proteins in this group are designated as follows: X, human TGIFLX; 1, mouse Tex1; M, human Meis2; P, human Prep1; C, C. elegans CEH-25; T, S. cerevisiae Tos8p. These represent two recently identified TGIF-related proteins—human TGIF-like on the X (X) and mouse Tex1 (indicated by 1)—the homeodomain from the two next-most-similar human TALE proteins (Meis2 and Prep1), and the most-similar homeodomain from S. cerevisiae and C. elegans. The positions of each of the three alpha helices are shown, together with the +20 region and the TALE. A “+” within helix 3 indicates the DNA contact residues. Amino acids that are identical or similar to human TGIF are shaded black or gray, respectively.