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. 2003 Dec;23(24):9262–9274. doi: 10.1128/MCB.23.24.9262-9274.2003

FIG. 3.

FIG. 3.

Zebra fish TGIF is a Smad transcriptional corepressor. (A) COS-1 cells were cotransfected with Flag-zTGIF and T7-CtBP expression vectors as indicated, and proteins were isolated on T7 agarose (immunoprecipitation [IP]) and analyzed by Western blotting (WB) for the presence of Flag-zTGIF. A portion of the lysates was analyzed by direct Western blotting for the presence of Flag-zTGIF and T7-CtBP (shown below). (B) COS-1 cells were cotransfected with Myc-Sin3 and Flag-tagged human or zebra fish TGIF expression vectors. Proteins were collected on Flag agarose and Western blotted for Myc-Sin3. Expression controls are shown below. (C) L17 cells were transfected with the 3TP-lux reporter with or without increasing amounts (30 or 100 ng per well) of a human or zebra fish TGIF expression vector. Cells were treated with TGF-β and assayed for luciferase activity 18 h later. Activity is presented as mean ± standard deviation of triplicate transfections in arbitrary units. The fold induction by TGF-β is shown for each condition.