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. 2003 Dec;23(24):9262–9274. doi: 10.1128/MCB.23.24.9262-9274.2003

FIG. 7.

FIG. 7.

mTex1 is a transcriptional repressor. (A) HepG2 cells were cotransfected with the (Gal)5-SV40 luciferase reporter and two amounts (5 or 30 ng) of expression vectors encoding GBD alone or fusions of full-length human or zebra fish TGIF to the GBD or aGBD-mTex1 fusion. Luciferase activity (mean ± standard deviation of triplicate transfections) is shown, together with that from cells without any GBD fusion. (B) Flag-tagged mTex1 was coexpressed in COS-1 cells with expression vectors encoding either Myc-tagged mSin3 or T7-tagged CtBP, and proteins were isolated on Flag agarose. Coprecipitating proteins are indicated by arrows, and expression in the lysates (assayed by direct Western blotting [WB]) is shown below. (C) Flag-tagged dTGIFa, or human TGIF as a positive control, was coexpressed in COS-1 cells with expression vectors encoding either Myc-tagged mSin3 or T7-tagged CtBP, and proteins were isolated on Flag agarose. Coprecipitating proteins are indicated by arrows, and immunoglobulin heavy chain is indicated by a line. Protein expression in the lysates was assayed by direct Western blotting and is shown below.