Figure 3.

Role of 14-3-3 binding in the nuclear distribution of Xwee1. A plasmid encoding GFP-Xwee1-N342A (top) or GFP-Xwee1-N342A-S549A (bottom) was transfected into XTC cells along with a plasmid encoding the Myc peptide alone (left) or Myc-14-3-3ε (right). The N342A and S549A mutations abolish kinase activity and 14-3-3 binding, respectively. The signal from GFP or DAPI was observed by fluorescence microscopy. The arrowhead in the top panel on the right indicates condensed chromosomes from a mitotic cell. Typically, we did not observe a GFP signal from mitotic cells.