FIG. 3.

RT-PCR analysis of β-like globin expression in erythroid cells of HS5 YAC transgenic mice. (A) Total RNA was prepared from the spleens of 1- to 2-month-old anemic mice and subjected to cDNA synthesis by reverse transcriptase (RT). The relative expression levels of the β-like globin genes, after normalization to that of the endogenous mouse α-globin gene, were determined by RT-PCR analysis. Data were collected from two individuals for each line and the average and standard deviation, determined by three sets of PCRs, are graphically depicted. Representative results of RT-PCR for human β (hβ) and mouse α (mα) amplicons are shown below each panel. (B and C) Total RNA was prepared from the liver (E14.5) or yolk sacs (E9.5) of two fetuses in two litters (lit.1 and lit.2) derived from the intercross of male transgenic and female wild-type animals. Representative results of RT-PCR for human ɛ (hɛ), γ (hγ), β (hβ), and mouse α (mα) are shown below each panel.