TABLE 3.
G1 escape and active DNA replication in normoxic and anoxic cellsa
| Cell line | Chase time (h) | % IdU−/CIdU+
|
|
|---|---|---|---|
| Normoxia | Anoxia | ||
| REF52/Bcl-2/pBabe | 4 | 23.5 | 2.8 |
| 8 | 30.1 | 6.0 | |
| REF52/Bcl-2/E1a | 4 | 17.0 | 15.2 |
| 8 | 27.5 | 14.9 | |
a
Cells were labeled and treated as described in the text. The percentages of cells that did not incorporate the first label, IdU, but did incorporate the second label, CIdU, are reported. At least 100 nuclei were counted for each experiment, and each experiment was repeated at least three times.