TABLE 2.
Flow cytometry analysis of reconstitution of hematopoietic lineages in irradiated mice by Siah2−/− and Siah2−/− Siah1a−/− fetal liver cellsa
| Organ and lineage marker | % Positive cells (mean ± SD) from mice
|
|
|---|---|---|
| Siah2−/− | Siah2−/− Siah1a−/− | |
| Thymus | ||
| CD45.2+ | 97.4 ± 0.5 | 93.9 ± 6.2 |
| CD4+ | 77.9 ± 2.3 | 75.9 ± 4.2 |
| CD8+ | 79.9 ± 1.1 | 78.3 ± 3.7 |
| Spleen | ||
| CD45.2+ | 90.1 ± 4.8 | 87.0 ± 1.3 |
| CD4+ | 18.3 ± 1.1 | 13.0 ± 0.5 |
| CD8+ | 6.2 ± 0.8 | 5.1 ± 1.1 |
| B220+ | 58.0 ± 1.2 | 59.2 ± 0.1 |
| Bone marrow | ||
| CD45.2+ | 70.9 ± 4.8 | 73.3 ± 4.3 |
| B220+ | 43.0 ± 5.5 | 35.9 ± 6.9 |
| Mac-1+ | 43.9 ± 6.7 | 46.8 ± 1.8 |
a
Data represent the results of an analysis of three recipient mice 19 weeks after adoptive transfer of fetal liver cells from Siah2−/− or Siah2−/− Siah1a−/− embryos and are percentages of total white blood cells that are derived from donor fetal livers (CD45.2+) and percentages of CD45.2+ cells that express the indicated T, B, and myeloid lineage markers.