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. 2003 Dec;23(24):9150–9161. doi: 10.1128/MCB.23.24.9150-9161.2003

TABLE 2.

Flow cytometry analysis of reconstitution of hematopoietic lineages in irradiated mice by Siah2−/− and Siah2−/− Siah1a−/− fetal liver cellsa

Organ and lineage marker % Positive cells (mean ± SD) from mice
Siah2−/− Siah2−/− Siah1a−/−
Thymus
    CD45.2+ 97.4 ± 0.5 93.9 ± 6.2
    CD4+ 77.9 ± 2.3 75.9 ± 4.2
    CD8+ 79.9 ± 1.1 78.3 ± 3.7
Spleen
    CD45.2+ 90.1 ± 4.8 87.0 ± 1.3
    CD4+ 18.3 ± 1.1 13.0 ± 0.5
    CD8+ 6.2 ± 0.8 5.1 ± 1.1
    B220+ 58.0 ± 1.2 59.2 ± 0.1
Bone marrow
    CD45.2+ 70.9 ± 4.8 73.3 ± 4.3
    B220+ 43.0 ± 5.5 35.9 ± 6.9
    Mac-1+ 43.9 ± 6.7 46.8 ± 1.8
a

Data represent the results of an analysis of three recipient mice 19 weeks after adoptive transfer of fetal liver cells from Siah2−/− or Siah2−/− Siah1a−/− embryos and are percentages of total white blood cells that are derived from donor fetal livers (CD45.2+) and percentages of CD45.2+ cells that express the indicated T, B, and myeloid lineage markers.

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