TABLE 3.
Histomorphometric analysis of bone structure and remodelling in Siah2 mutant micea
| Parameter | Mean ± SD
|
|
|---|---|---|
| Wild type mice | Siah2−/− mice | |
| Femoral length (mm) | 14.5 ± 0.3 | 14.3 ± 0.2 |
| Femoral width (mm) | 1.45 ± 0.10 | 1.31 ± 0.05 |
| Cortical thickness (μm) | 101 ± 3 | 97 ± 6 |
| Trabecular bone volume (%) | 6.9 ± 2.4 | 5.9 ± 1.1 |
| Trabecular no. (per mm) | 2.0 ± 0.5 | 2.0 ± 0.3 |
| Trabecular thickness (μm) | 28.8 ± 4.0 | 28.0 ± 2.4 |
| Trabecular separation (μm) | 686 ± 184 | 571 ± 105 |
| Osteoblast surface (%) | 25.3 ± 2.0 | 31.5 ± 3.4 |
| Osteoid vol/bone vol (%) | 3.8 ± 0.8 | 3.7 ± 0.7 |
| Osteoid thickness (μm) | 2.3 ± 0.2 | 1.8 ± 0.3 |
| Mineral appositional rate (μm/day) | 2.5 ± 0.6 | 1.9 ± 0.1 |
| Mineralizing surface (%) | 14.0 ± 3.2 | 19.9 ± 3.6 |
| Osteoclast surface (%) | 10.2 ± 0.9 | 13.1 ± 2.0 |
| Osteoclast no. (per mm of BPM) | 3.0 ± 0.3 | 3.5 ± 0.5 |
a
Data are means ± standard errors of the means from an analysis of metaphyseal regions of tibiae from 8-week-old wild-type (n = 8) and Siah2−/− (n = 7) mice. No statistically significant difference among mice of different genotypes was found for any parameter (by ANOVA and Fisher's post hoc test). BPM, bone perimeter.