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Intact prepubertal male and female mice exhibit similar numbers of Kiss1 neurons in the ARC. However, gonadectomized prepubertal females have higher ARC Kiss1 expression than similarly-aged gonadectomized males. The absence of elevated Kiss1 expression in gonadectomized prepubertal males suggests that some non-gonadal factor(s) acts to suppress the ARC Kiss1 system in males at this developmental stage. (3V = 3rd ventricle; OVX = ovariectomized; cast= castrated)
